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ディテクターから出力された、電気信号を記録し、そこからピークを検出、解釈を行う。結果は、感熱紙等に印字される。装置のコントロールをしないのであれば、どのメーカーの物を使用しても問題はないが、通常は、装置のコントロールも同時に行うため、同じメーカーの物を選択する。
The realm of the height is routinely detected by the computer. The pc also detect the retention time of that specific part.
, which lets us to investigate a wide variety of mobile phases with only 7 experiments. We commence by altering the amount of acetonitrile from the cellular section to generate the absolute best separation in just the desired analysis time.
). In case the detector is usually a diode array spectrometer, then we also can display The end result as a three-dimensional chromatogram that demonstrates absorbance for a operate of wavelength and elution time.
이러한 특징으로 고성능 액체 크로마토그래피는 전 세계 모든 과학 분야 및 산업의 기반을 뒷받침하는 과학기술로서의 위치를 확립하고 있습니다.
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It achieves this by exploiting the differing interactions of sample compounds with two essential phases: the cellular section and also the stationary section. Knowledge the Main elements of an HPLC system as well as their roles is important for effective Evaluation.
Differing types of detectors Utilized in HPLC are refractive index detectors, UV detectors, and fluorimetry detectors.
Normal-phase: Separates based upon polarity. Analytes with higher polarity interact a lot more Along with the polar stationary stage and elute later on.
The column may be the separation chamber exactly where the magic of HPLC comes about. It houses the stationary stage, a packed mattress of microscopic particles.
, a fluorescence detector supplies supplemental selectivity since only some of a sample’s parts are fluorescent. more info Detection limits are as little as one–10 pg of injected analyte.
Immediately after loading the sample, the injector is turned into the inject posture, which redirects the cell stage through the sample loop and onto the column.
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